Substrates for Bioanalysis & Cell Culture
Bioarrays enable high-throughput molecular analyses and have aided tremendous advances in life science research. Further increasing bioarray sensitivity and reproducibility will enable broader acceptance and standardization for more demanding settings, such as clinical diagnostics. Furthermore, increasing the range of options in bioarray substrates and surface chemistries - key elements in performance - will enable greater design flexibility and novel applications, especially with respect to protein and glycan-based arrays.
Several formats of the monolithic 3-D nanoporous bioarray substrate.
Synkera is developing novel bioarray substrates that can significantly enhance bioarray performance through a unique 3-D nanoporous architecture that features high specific surface area and flexible surface chemistry. These substrates can serve as drop-in replacement for current glass-based arrays as well as support novel bioarrays and assays.
Substrate features and benefits
- High density 3-D spots due to high surface area, self-organized architecture of nanoporous anodic alumina.
- Precise high-resolution spotting due to vertical pore alignment.
- Nanopore size is precision controlled in the 10 to 300 nm range for fine-tuning assay performance.
- Compatible with traditional surface-based assays, as well as with advanced flow-through modes for faster assays.
- Nanoporous sites can be patterned for precise array spotting (spot size as low as 1 µm).
- Various surface chemistry options, including that similar to glass substrates, via conformal modification of nanopores.
- Micromachinable for integration with advanced biochips (e.g. microfluidics).
Status
SEM of the cross-section (left) and the surface (right) of the array featuring 50 nm diameter nanopores. Note that the surface of the array is patterned for localized spotting.
Leveraging its extensive capabilities in membranes and microcomponents from nanoporous ceramics, Synkera completed initial development and now offers Gen-1 substrates. These substrates are being evaluated for fluorescence-based assays and other bioanalysis applications. You can see our current offerings and get further info by fulling out an Info/Quote Request form.
Partnership Development
Partners and customers are being sought for developing specific applications of these substrates. If you need additional information, are interested in prototype evaluation, or would like to discuss this further, please email us.